pegfp c3 hyap1 (Addgene inc)

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Addgene inc pegfp c3 hyap1
Pegfp C3 Hyap1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c3+hyap1/pEGFP-C3-hYAP1+(Plasmid+%2317843)/pmc13000468-37-64-71
Average 94 stars, based on 57 article reviews

pegfp c3 hyap1 - by Bioz Stars, 2026-09

94/100 stars

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Plasmid Preparation:

Article Title: Regulation of YAP activity by nuclear G-actin binding
Article Snippet: .. pET-41 GST-Rpel (MRTF-A) and pRLTK-Renilla-Luc were a gift from Treisman Richard [ ]. pCMV-flag YAP2 5SA was a gift from Kunliang Guan (Addgene plasmid # 27 371; http://n2t.net/addgene:27371; RRID: Addgene_27 371) [ ]. pGL3b-8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34 615; http://n2t.net/addgene:34615; RRID: Addgene_34 615) [ ]. pEF Flag NLS actin was a gift from Dr Guido Posern [ ]. pEGFP-C3-hYAP1 was a gift from Marius Sudol (Addgene plasmid # 17 843; : 17 843; RRID: Addgene_17 843)[ ]. pLKO1-shYAP1 was a gift from Kunliang Guan (Addgene plasmid # 27 368; : 27 368; RRID: Addgene_27 368) [ ]. ..

Article Title: Regulation of YAP activity by nuclear G-actin binding.
Article Snippet: .. pET -41 GST -Rpel (MRTF-A) and pRLTK-Renilla-Luc were a gift from Treisman Richard [ 15 ]. pCMV-flag YAP2 5SA was a gift from Kunliang Guan (Addgene plasmid # 27 371; http:// n2t.net/ addgene:27371; RRID: Addgene_27 371) [ 21 ]. pGL3b-8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34 615; http:// n2t.net/ addgene:34615; RRID: Addgene_34 615) [ 22 ]. pEF Flag NLS actin was a gift from Dr Guido Posern [ 38 ]. pEGFP-C3-hYAP1 was a gift from Marius Sudol (Addgene plasmid # 17 843; : 17 843; RRID: Addgene_17 843)[ 39 ]. pLKO1-shYAP1 was a gift from Kunliang Guan (Addgene plasmid # 27 368; : 27 368; RRID: Addgene_27 368) [ 40 ]. ..

Article Title: SOX5 inhibition overcomes PARP inhibitor resistance in BRCA-mutated breast and ovarian cancer.
Article Snippet: Plasmids and ectopic overexpression studies The SOX5 overexpression vector, (cat# RC224228) and the respective empty vector (cat# PS100001) were purchased from Origene (Rockville, MD, USA). .. The pEGFP-C3-hYAP1 (Addgene plasmid #17843) overexpression vector was purchased from Addgene (Watertown, MA, USA). .. Transfection was performed through CalFectinTM Mammalian DNA transfection reagent (cat# SL100478, SignaGen Laboratories, Frederick, MD, USA) according to the manufacturer’s protocol with little modification.

Article Title: Mechanics-dependent Global Nuclear Eviction and Site-Specific Recruitment of YAP Regulates DNA Damage Responses
Article Snippet: .. The following plasmids were used in this study: pEGFP-C3-hYAP1 (Addgene plasmid # 17843), pDsRed Monomer C1-YAP2 (Addgene plasmid # 19057) and pDsRed Monomer C1-YAP2-S127A mutant (Addgene plasmid # 19058) was a gift from Marius Sudol. .. OptoYAP (pDN34-BiNLS-hYAP1-1delta) was a gift from Timothy Saunders (Addgene plasmid # 169471; http://n2t.net/addgene:169471 ; RRID: Addgene_169471). mApple-dnNesprin1 (amino acids 8369-8749 of human Nesprin-1 cloned into mApple-C1) (DOI:10.1242/jcs.02471), mApple-dnLaminB1 or XLaminB1Δ2+ (34-420 aa from X06344 cloned into mApple-C1) (PMID: 11683386) and C1-mApple (Addgene #54631) was a gift from Tamal Das.

Article Title: Heart‐on‐a‐Miniscope: A Miniaturized Solution for Electrophysiological Drug Screening in Cardiac Organoids
Article Snippet: .. NIH 3t3 cells were cultured to a confluency of 90% and then were transfected with pEGFP‐C3‐hYAP1, a gift from Marius Sudol (Addgene plasmid # 17 843) for 12 h. 40 hours post‐transfection, the medium was changed to complete phenol‐free DMEM and cells were imaged with the LWD Miniscope in a humidified chamber set at 37 °C and 5% CO 2 . .. The osmotic pressure was applied using 10% v/v solution of Polyethylene glycol 400 (#1 546 445, Millipore Sigma) in culture media.

Article Title: Regulation of YAP activity by nuclear G-actin binding
Article Snippet: .. pET-41 GST-Rpel (MRTF-A) and pRLTK-Renilla-Luc were a gift from Treisman Richard ( ). pCMV-flag YAP2 5SA was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371 ; RRID:Addgene_27371) ( ). pGL3b-8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34615; http://n2t.net/addgene:34615 ; RRID:Addgene_34615)( ). pEF Flag NLS actin was a gift from Dr. Guido Posern ( ). pEGFP-C3-hYAP1 was a gift from Marius Sudol (Addgene plasmid # 17843; http://n2t.net/addgene:17843 ; RRID:Addgene_17843)( ). pLKO1-shYAP1 was a gift from Kunliang Guan (Addgene plasmid # 27368; http://n2t.net/addgene:27368 ; RRID:Addgene_27368)( ). ..

Article Title: SOX5 inhibition overcomes PARP inhibitor resistance in BRCA-mutated breast and ovarian cancer
Article Snippet: The SOX5 overexpression vector, (cat# RC224228) and the respective empty vector (cat# PS100001) were purchased from Origene (Rockville, MD, USA). .. The pEGFP-C3-hYAP1 (Addgene plasmid #17843) overexpression vector was purchased from Addgene (Watertown, MA, USA). .. Transfection was performed through CalFectinTM Mammalian DNA transfection reagent (cat# SL100478, SignaGen Laboratories, Frederick, MD, USA) according to the manufacturer’s protocol with little modification.

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Article Title: Spatiotemporal distribution of YAP and importin-β in nucleus and cytosol after wounding of adjacent cells.
Article Snippet: Yes-associated protein (YAP), an important downstream mediator of the Hippo pathway, exhibits nuclear translocation when it is dephosphorylated.. However, its interplay with other intracellular substances has not yet been clarified.. Here, we explored mechano-induced YAP nuclear translocations and its interplay with importin-β (Impβ), one of the nucleocytoplasmic transport receptors, through live imaging of the spatiotemporal distribution of intracellular YAP and Impβ following mechanical stimulation, represented by the release of intercellular tension.

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